| 摘要: |
| 以PCR、免疫荧光、细胞毒试验在DNA、表达、功能水平对转hDAF基因猪进行了检测。结果表明转基因猪的整合率为19.35%(18/93)；在14头转基因阳性猪中，有7头动脉肌肉层表达了外源hDAF基因，6头猪的外源基因表达产物可以抑制猪淋巴细胞的裂解。 |
| 关键词: 检测系统 转基因猪 hDAF |
| DOI： |
| 分类号:Q789 |
| 基金项目:国家“863”项目(101-05-05-01) |
|
| The foundation of xenotransplantation transgenic pig detection system |
|
YIN Ming an 1,CUI Hong wen 1,FAN Dai ming 2,GUO Li 3,YIN Ming an 1,CUI Hong wen 1,FAN Dai ming 2,GUO Li 3,YIN Ming an 1,CUI Hong wen 1,FAN Dai ming 2,GUO Li 3
|
| Abstract: |
| By using PCr,immunfluorescence,microlymphocytotoxicity,transgenic pigs were detected in levels of DNA,expressing and function.The results indicated that the integration ratio was 19.35%(18/93).Of the 14 transgenic pigs,7 pigs’vascular smooth muscle expressed exogene of hDAF,and the expressing products of hDAF in 6 transgenic pigs could delay pigs’lymphocyte cleavaging. |
| Key words: detection system transgenic pigs hDAF |
