| 摘要: |
| 苦皮藤素Ⅴ是从杀虫植物苦皮藤 (Celastrus angulatus Max)根皮提取物中分离得到的对昆虫有毒杀活性的一种新化合物。通过对该化合物引起粘虫产生类似神经毒剂兴奋性症状的机理研究 ,结果表明 ,苦皮藤素 对昆虫中枢神经系统兴奋性突触后电位(EPSP)和轴突动作电位 (AP)无显著影响 ;试虫经苦皮藤素Ⅴ处理后 ,抽搐期和失水期体内乙酰胆碱酯酶 (ACh E)活性分别下降 33.9%和 39.6% ,谷氨酸 (Glu)含量分别升高 2 8.44%和 49.18% ,γ-氨基丁酸 (GABA)含量分别降低 42.1 1 %和 44.47%。抽搐期体内谷氨酸脱羧酶活性降低了 8.67%。 |
| 关键词: 植物性杀虫剂,苦皮藤素,作用机理,电生理 |
| DOI： |
| 分类号:S482.39 |
| 基金项目:国家自然科学基金资助项目(29522003) |
|
| Study on themechanism of action of celangulin Ⅴ |
|
YANG Run ya,LIU Hui xia,HU Zhao nong,ZHANG Lin sheng,TIAN Xiao man,YANG Run ya,LIU Hui xia,HU Zhao nong,ZHANG Lin sheng,TIAN Xiao man,YANG Run ya,LIU Hui xia,HU Zhao nong,ZHANG Lin sheng,TIAN Xiao man
|
| Abstract: |
| This paper preliminarily reports the mechanism of generating excitation as
neurous insecticides of Celangulin Ⅴ isolated from the root part of Celastrus angulatus
Max, using electrophysiological and biochemical methods. The electrophysiologica
results show that Celangulin Ⅴ has no effects on the excitatory postsynaptic potentia
(EPSP) and the action potential of the nerve-giant fiber axon from the cockroach CNS
preparation. After the sixth-instar larvae of Mythimna separata following oral
administration with Celangulin Ⅴ, the activity of acetyl choline esterase (AChE) of
excitatory and convulsionary larvae decreased by 33. 9% and 39. 6% respectively,
compared with normal larvae; the glutamate (Glu) content raised by 28. 44% and
49. 18% and the content of γ-aminobutiric acid (GABA ) reduced by 42. 11% and
44. 47%. In the convulsionary larvae, the activity ofglutam ic decarboxylase decreased by
8. 67%. |
| Key words: botanical insecticide Celangulin Ⅴ mechanism of action elctrophysiology |
