| 摘要: |
| 按照肝脏受TAA损伤的程度把试验动物Wistar大白鼠分为两组(TAA-1和TAA-2)。Northern杂交分析表明,在 TAA-1的窦内皮细胞中,bcl-x和bax基因的表达水平轻微降低;随着肝损伤的增加,表达水平再度下降。核Run-off转录分析表明,两组的窦内皮细胞、总的 RNA合成率下降约30%(P<0.001);TAA-1、bad、bax和cyclin E的mRNA合成有所降低,而cyclin A的mRNA合成则明显增加;TAA-2、bad、bax和bcl-2的mRNA合成增加了2～3倍 ,bcl-x的mRNA合成增加了7倍多。 |
| 关键词: 硫代乙酰胺(TAA),肝纤维化,窦内皮细胞,凋亡基因,周期基因,基因表达 |
| DOI： |
| 分类号:Q344.13 |
| 基金项目:德国DFG基金资助项目 |
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| Study on the expression of genes in sinusoidal endothelial cells of rat liver with fibrogenesis |
|
CHEN Hong 1 F M Stolz 2 R Dargel 2
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| Abstract: |
| Experimental animals of Wistar rats using oral treatment of Thioacetamide (TAA) were divided into two groups (TAA-1 and TAA-2) according to the extent of liver injury.The analysis by means of Northern blotting showed that a slightly decreased expression level of bcl-x and bax was observed in sinusoidal endothelial cells (SEC) of group TAA-1.In SEC of group TAA-2,a further decreased expression of bcl-x and bax was more slightly detected.The analysis of nuclear run off transcription assays showed that,in both experimental groups,the rate of incorporation of 32P-UTP into total RNA by isolated nuclei of SEC was significantly reduced by about 30% (P<0.001) and that the synthesis of mRNA of bad,bax and cyclin E was moderately decreased in SEC of the early phase of liver injury,while that of cyclin A and bcl-x was significantly increased.In the late phase of TAA induced fibrogenesis (TAA-2),the synthesis of mRNA of bad,bax and bcl-2 in SEC was increased by 2-3 fold and that of bcl-x was raised more than 7 fold. |
| Key words: thioacetamide,liver fibrogenesis,sinusoidal endothelial cells,apoptosis-genes,cyclin-genes,gene expression |
