| 摘要: |
| 外源大赖草DNA直接导入普通小麦,诱导小麦籽粒蛋白质组成及过氧化物酶、酯酶、超氧化物歧化酶同工酶谱带发生了显著变化，蛋白质分别增加44，67，85ku新组分，蛋白质和氨基酸含量分别提高16.82%,15.62%，赖氨酸增长42.94%，同工酶不同程度出现了差异谱带，酶带缺失，酶活性增减的明显变化。导入DNA的变异小麦的籽粒色泽、硬度、胚乳粉质等生物学性状都有一些改变，其变异现象在连续后代中依然表达。 |
| 关键词: 小麦 大赖草DNA 外源DNA导入 蛋白质 同工酶 |
| DOI： |
| 分类号:Q343.1 |
| 基金项目:陕西省自然科学基金资助项目(99SM05) |
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| An analysis of variation characteristics of the progenies derived from introducing leymus racemosus gene into common wheat |
|
ZHU Xin chan,LIAO Xiang ru,FU Zheng guang,ZHU Xin chan,LIAO Xiang ru,FU Zheng guang,ZHU Xin chan,LIAO Xiang ru,FU Zheng guang
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| Abstract: |
| Exogenous DNA directly introduced into wheat plant induced striking change in seed protein components of wheat and isozymogram of peroxidase isozyme (POD),esterase isozyme (EST) and superoxide dismutase (SOD).The proteins increased by 44 ku,67 ku,85 ku new compoents,and the protein and amino acid contents enhanced by 16.85% and 15.62% separately,Lys increased by 42.94%.Isozyme also showed difference isozyme bands,losing isozyme bands and increase and decrease of enzyme activity in varying degrees.Some bio characteristics of variant grains derived from introducing exogenous DNA into common wheat,such as colour,hardness,endosperm trasparency etc,also showed remarkable changes.These variations can be stably expressed in successive progenies. |
| Key words: wheat,leymus recemosus gene,exogenous DNA introduction,protein,isozyme |
