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山羊冰川棘豆中毒的血清蛋白分析
耿果霞1, 李勤凡1, 王建华1
西北农业大学 动物科学与动物医学学院
摘要:
将10只杂种奶山羊随机分为试验组和对照组,每组5只,试验组羊按10 g·kg-1·d-1的剂量饲喂冰川棘豆草粉,在试验的第0, 7, 14, 21, 28, 35, 42, 49天,分别测定血清总蛋白、球蛋白、白蛋白,并进行血清蛋白电泳分析。结果表明,血清总蛋白、球蛋白、α球蛋白和β球蛋白均无明显变化,而γ球蛋白从试验的第14天起持续下降,白蛋白在试验初期降低,而在试验后期无明显变化;白蛋白/球蛋白值(A/G)低于对照组,与对照组比较差异显著(P<0. 05或P<0. 01),说明中毒羊肝、肾等器官受损,体液免疫功能降低。
关键词:  冰川棘豆,中毒,血清蛋白,山羊
DOI:
分类号:S452*
基金项目:陕西省“西藏阿里地区草原毒草防除研究”课题子项目(0060C8)
Analyses of serum protein by experimental poisoning of oxytropis glacialis in dairy goats
Abstract:
Ten goatswere randomly divided into two groups (test and control, 5 per group), and fed the diet supplemented with Oxytropis glacialis at the level of 10 g·kg-1·d-1 for test group. During 0, 7, 14, 21, 28, 35, 42 and 49 days of feeding, serum total protein, globulin and albumin of each group were measured respectively. Electrophoresis analysiswas donewith serum protein The results indicated that serumγ globulin was significantly dropped (P<0. 01) from 14 d of feeding, the ratio of albumin and globulin was significantly decreased (P< 0. 05 or P< 0. 01), also dropped for albumin at early stage. Serum total protein, globulin,α-globulin and β-globulin were no significantly difference (P>0. 05).These results showed that lives and kidney and other viscera were damaged and the function of humoral immunity was decreased by poisoning of Oxytropis glacialis.
Key words:  Oxytropis glacialis,poisoning,serum protein,goat