| 摘要: |
| 用限制性内切酶将抗虫的胰蛋白酶抑制剂（TＩ）基因从质粒pBin20上切下，插入带有抗细菌的抗菌肽B和抗菌肽Ｄ基因的植物表达载体pDB3的XbaⅠ位点。通过点杂交和酶切鉴定，得到了带有3个抗性基因的双抗植物表达载体pDBT． |
| 关键词: 胰蛋白酶抑制剂基因，抗菌肽B基因，抗菌肽D基因，载体构建 |
| DOI： |
| 分类号:S6 |
| 基金项目:热带作物生物技术国家重点实验室资助项目 |
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| Construction of Plant Expression Vector with Double Resistance to Bacteria and Insect |
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| Abstract: |
| A plant expression vector with resistance to both bacteria and insect was constructed by inserting a trypsin inhibitor gene cut from pBin20 with restriction endonuclease into XbaⅠ site of pDB3 containing cecropin B and cecropin D genes.Dot blotting and restriction enzyme digestion analysis showed recombinant pDBT containing three genes was obtained. |
| Key words: trypsin inhibitor gene,cecropin B gene,cecropin D gene,construction of plant expression vector |
