| 摘要: |
| 采用外周血淋巴细胞培养法及常规染色技术,以TdR和FUdR作诱导剂,对家猪染色体脆性位点进行初步研究。结果显示,TdR诱导家猪脆性位点的有效处理浓度为60～80mg·L-1,脆性位点的表达频率为65%左右。TdR型脆性位点主要发生于1q, 2q, 6q, 13q,16q染色体,FUdR型脆性位点主要集中于1q, 2q, 6q, 13q, 14q, 15q, 16q染色体,家猪的脆性位点表达可能存在个体差异。并对脆性位点的类型及生物学意义进行了简要讨论。 |
| 关键词: 家猪,染色体,脆性位点 |
| DOI： |
| 分类号:S828.2 |
| 基金项目:国家自然科学基金资助项目（0594） |
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| A Primary Study on Fragile Sites in Domestic Pig Chromosome |
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| Abstract: |
| By using the peripheral blood lymphocyte culture method and common staining technology in this experiment,a primary study on fragile sites in domestic pig chromosome is done with Thymidine (TdR) and Fluorodeoxyuridine (FUdR) as inducer.The result shows that a concentration of 60-80 mg of TdR L-1 of medium is recommended as the optimum for fragile sites expression in domestic pig and the expression rate of fragile sites is about 65 percent.The TdR-sensitive fragile sites in domestic pig are identified on Chromosomes 1q,2q,6q,13q and 16q,whereas the FUdR-sensitive fragile sites are identified on Chromosomes 1q,2q,6q,13q,14q,15q and 16q.Individual differences may exist in the expression of fragile sites in domestic pigs.The classification and biological significance of fragile sites are also briefly discussed. |
| Key words: domestic pig,chromosome,fragile site, |
