| 摘要: |
| 以方阵滴定法选择出最适ELISA反应条件,用系列稀释法测定21份血清样品的禽霍乱抗体ELISA效价(ET),并与相应血清样品1∶200倍稀释的P/N值配对,作线性回归分析,得到回归方程y=219. 673a-183. 570(r= 0. 944)和标准曲线,从而建立起快速定量测定禽霍乱抗体的间接ELISA方法——阳阴比值ELISA效价法(P/NI-ET法)。利用该法,血清样品只需作1∶200倍稀释,测定其P/N值,通过回归方程(或标准曲线)即可求得ET. |
| 关键词: 间接ELISA，P/N-ET法，禽霍乱抗体 |
| DOI： |
| 分类号:S852.53,S854.43 |
| 基金项目:西北农业大学青年科学基金资助项目 |
|
| Establishment of Indirect ELISA for Detecting Fowl Cholera Antibody |
|
|
| Abstract: |
| Using the method of chessboard titration to choose the proper reaction
condition, after a series of dilution, 21 samples of chicken serum were detected for their
fowl cholera (FC) antibody ELISA titer (ET).Paired with ET and the P/N value of 1:200 dilution and after linear regression analysis, a linear equation y=219. 673x-183. 570 (r= 0. 944) and a standard diagram of curves were got.Thus an indirect ELISA
which could rapidly and quatitatively detect FC antibody-P/N-ET method was established,with which the ET could be canculated out with regression equation only if the
serum samples were diluted at 1∶200 and P/N value determined. |
| Key words: indirect ELISA,P/N-ET, fowl cholera antibody |
