摘要: |
用电导法测定了小麦叶片经Gn-粗毒素处理后的组织细胞膜损伤情况,发现毒素能导致小麦叶片细胞内电解质外渗,不同抗性品种对毒素反应有明显差异,抗病品种的细胞损伤率明显低于感病品种。电镜观察发现,毒素还引起组织超微结构发生显著变化,包括叶绿体膜上出现电子致密度高的沉积物,基粒片层肿胀,排列紊乱;直至叶绿体、细胞核和整个细胞解体。损害发生最早最严重的是叶绿体基粒片层。抗病品种比感病品种的超微结构受害轻、反应迟。 |
关键词: 小麦雪霉叶枯病,雪腐格氏霉,毒素,细胞膜,超微结构 |
DOI: |
分类号:S432.1,Q949.329.30 |
基金项目:国家“九五”攻关项目 |
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The Effect of Crude Toxin from Gerlachia nivalis on the Membrane Permeability and Ultrastructure of Wheat Leaf |
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Abstract: |
The damage of wheat cell membrane treated with crude toxin is examined by electrical conductivity method and it is found that the toxin can damage wheat
leaf tissue cell membrane and cause its leakage of electrolytes in the cell, but there is an
obvious difference to toxin for different resistant cultivars.The cell damage rate of the
resistant cultivars is lower than that of susceptible cultivars. It has been also observed
that toxin results in the obvious change in ultrastructure of wheat leaf, including dilated,
disorganized and vacuolated grana lamellae, vacuolated mitochondrion and high density
electron deposition located on the chloroplast membrane. The chloroplast, nuclear and
cell collapse successively. The damage of chloroplast occurs earliest and seriously. The
effect of toxin on ultrastructure is heavier and faster on susceptible cultivars than on resistant cultivars. |
Key words: gerlachia leaf blight of wheat,Gerlachia nivalis,toxin,membrane,ultrastructure, |