| 摘要: |
| 本研究主要目的是应用死精子微注射法探索一种提高牛成熟卵母细胞体外受精的方法。试验划分为三个部分:试验1体外成熟卵母细胞分为4个处理组。1组和3组成熟卵母细胞分别用1μmoL·L-1和5μmoL·L-1的钙离子A23187激活; 2组和4组卵母细胞在1μmoL·L-1和5μmoL·L-1的钙离子A23187激活前去除颗粒细胞。精子获能用40mg·L-1肝素。在试验2和试验3,成熟卵母细胞的激活方法和颗粒细胞的去除同试验1,精子获能分别用50mg·L-1和100mg·L-1肝素。当卵和精子经上述处理后,一个获能后的死精子微注射到卵细胞质中。试验结果表明:体外成熟的牛卵母细胞,当用5μmoL·L-1钙离子A23187激活后,经死精子显微注射,受精率明显地高于1μmoL·L-1的钙离子激活组。当精子获能用不同剂量的肝素处理后,对牛体外成熟的卵母细胞受精率未产生明显的影响。在死精子显微注射受精过程中,当成熟牛卵细胞在钙离子激活前去除颗粒细胞将明显地降低其受精率。 |
| 关键词: 牛，卵母细胞，精子，显微注射 |
| DOI： |
| 分类号:S823.335 |
| 基金项目: |
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| Fertilization of Bovine Oocytes by Spermatozoal Microinjection |
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| Abstract: |
| The objective of this study was to explore amethod to increase the fertilization rate of bovine oocytes matured in vitro by the microinjection of a single immotile spermatozoon. The experiment was divided into three parts. In experiment 1, matured oocytes were
activated for four treatments with 1μmoL·L-1, 1μmoL·L-1(cumulus cells were removed
before activated), 5μmoL·L-1 and 5μmoL·L-1(cumulus cells were removed before activated) calcium ionophore A23187, respectively.Spermatozoa were capacitated by 40mg·L-1
heparin in four treatments. In experiments 2 and 3, matured oocytes were treated like experiment 1. Spermatozoa were capacitated by 50 mg·L-1 and 100 mg·L-1 heparin. After
oocytes and spermatozoa were treated, a single immotile spermatozoon was injected into the
ooplasm. The study results demonstrated that the fertilization rate of bovine oocytes matured
in vitro increased w ith 5μmoL·L-1 calcium ionophore A23187 by the immotile sperm injection compared with other treatments. The different concerntration heparin did not affect the
fertilization rate of bovine oocytes for sperm capacitation.Cumulus cells were romoved before
activated, which seriously reduced the fertilization rate of bovine oocytes by the microinjection of an immotile spermatozoon. |
| Key words: bovine,oocyte,spermatozoa,microinjection |
