| 摘要: |
| 对植物组织中蚜虫口针分泌的胶状唾液染色观察发现,蚜虫刺探时口针穿行在细胞间隙,仅在吸食时下颚口针进入细胞内。禾谷缢蚜饲毒12 h时RT-PCR方法在后肠、血淋巴液和附唾液腺中检测到GPV;而非介体麦长管蚜在GPV毒源上吸食24 h时,仅在后肠组织中检测到GPV;当继续取食48 h时,在后肠及血淋巴液中也发现了GPV,但仍无能力进入附唾液腺,说明蚜虫后肠和附唾液腺对病毒均有专化选择作用,禾谷缢管蚜传播BYDV-GPV时存在吸食、获毒和传毒三个阶段。 |
| 关键词: 大麦黄矮病毒，麦蚜传播，RT－PCR检测 |
| DOI： |
| 分类号:S435.12 |
| 基金项目:国家攀登计划与植物病虫害生物学国家重点实验室基金资助项目 |
|
| Mechanism of the Circulative Transmission of Barley Yellow Dwarf Letuovirus |
|
|
| Abstract: |
| When Rhopalosiphum padi and sitobion avenae feed on GPV-infected
oats, stylet mainly passes through intercellula rpath of tissue.Then a pair of maxillar only inserts phoem cell to feed sap by observation of saliva sheath stained with 0. 3%
Coomassie brilliant blue.R. padi requires GPV and GPV virions present in hindgut and
hemolymph by feeding on virus oats for 8h, and GPV virions exist in accessory salivary
gland (ASG) after feeding 12 to 24h, but only in hindgut and hemolymph of nonvector
S.avenae after feeding 48h by RT-PCR method. |
| Key words: barley yellow dwarf letuovirus, aphid transmission,RT-PCR analysis |
