摘要: |
用电镜细胞化学定位技术,对ms(Ae,kotschyi)-77(2)雄性不育花药及其保持系花药壁四层组织和维管组织细胞中的细胞色素氧化酶进行了定位和相对定量的研究。结果表明,药壁中层、绒毡层细胞内细胞色素氧化酶的变化,不育系与其保持系基本一致,而药壁表皮、药室内壁和维管组织细胞内线粒体中的细胞色素氧化酶的含量明显低于其保持系。推测,细胞色素氧化酶活性降低可能是导致花粉败育的重要因素之一。 |
关键词: 小麦,雄性不育,细胞色素氧化酶,超微结构 |
DOI: |
分类号:Q-336 |
基金项目:陕西省自然科学基金资助项目 |
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Cytochrome Oxidase Distribution of Anther Wall Cells from Male Sterile Line |
Yao Yaqin 1 Zhang Gaisheng 2
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Abstract: |
The results obtained by the cytochemical localization of cytochrome oxidase activity reaction indicate that reaction products of cytochrome oxidase activity in tapetal and middle-layer cells of anther wall from male sterile line,K-77(2)A,were similar to those from its maintainer line,77(2)B,but the reaction products in pidermis and endothecium cells of anther wall and in the vascular cells of anther from K77(2)A were far lower than those in 77(2)B.It is supposed that reduction oxidase activity was one of the causes resulting in wheat pollon abortion. |
Key words: wheat,male sterile,cytochrome oxidase,ultrastructure |