| 摘要: |
| 分别用不同条件的电脉冲、酒精浓度刺激不同卵龄的小鼠卵母细胞,进行体外培养并观察卵母细胞的活化和体外发育情况。结果表明,①以场强55 V /mm,脉宽160μs的电脉冲刺激卵龄为17 h的卵母细胞,活化卵形成二原核率为90%;以场强55 V /mm,脉宽640μs的电脉冲刺激卵龄为15 h的卵母细胞,激活卵的桑椹胚最高发育率为69%.②用7%酒精刺激卵龄为15 h的卵母细胞2min,活化率最高为72%,激活卵的桑椹胚发育率为22. 2%. |
| 关键词: 卵母细胞，电脉冲，酒精，孤雌生殖，小鼠 |
| DOI： |
| 分类号:S814.6 |
| 基金项目: |
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| Parthenogenetic Activation of Mouse Oocytes and in Vitro Development |
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| Abstract: |
| In this paper, mouse oocytes with different ages were stimulated by different electrical pulses and alcoholic concentrations and cultured in vitro. And their activation and development were observed. The results were as follows:①the oocytes of
17 h of age stimulated by electrical pulse with the duration of 160μs, field strength of
55V /mm formed 2 pronucleus with the activated oocyte rate of 90%, while the oocytes
of 15 h of age stimulated by electrical pulse with the duration of 640μs, field strength of
55V /mm could have the rate of the morulas development reach 69%;②stimulated by
7% alcoholic concentration for 2 minutes, the activation rate of 15 h oocytes was 72%
and the rate of the morulas developmentw as 22. 2%. |
| Key words: oocyte,electrical pulse,ethanol,parthenogenesis,mouse |
