| 摘要: |
| 通过硫酸铵分级分离和Sephadex G-100柱纯化得到的小麦叶片蛋白,可分为两个部分,其对硝酸还原酶(NR)都有明显的钝化活力;且钝化蛋白部分Ⅱ的钝化活力明显高于同样蛋白含量的部分Ⅰ.小麦叶片中硝酸还原酶钝化蛋白部分Ⅰ和Ⅱ同样对其根中的NR具有钝化活性。钝化蛋白部分Ⅰ通过聚丙烯酰胺凝胶电泳进一步纯化,达到电泳纯,测得分子量为47.5 kD. |
| 关键词: 硝酸还原酶，钝化蛋白，冬小麦 |
| DOI： |
| 分类号:Q554.9 |
| 基金项目:国家自然科学基金资助课题 |
|
| Purification and Activity of NR-Inactivating Proteins in Winter Wheat Leaf |
|
He Ping Wen Shuji Wang Peihong,He Ping Wen Shuji Wang Peihong,He Ping Wen Shuji Wang Peihong
|
| Abstract: |
| When a crude extract from wheat leaves was fractionated by a combination of ammonium sulfate (30%～45% saturation) precipatation and Sephadex G-100
filtration,the presence of two NR-inactivating proteins was revealed.Both inactivating
protein Ⅰ and inactivating protein Ⅱ had a strongly inhibatory effect on nitrate reductase activity from the leaves.The effect of inactivating protein Ⅱ was much higher than
that of protein Ⅰ when an equal amount of inactivating protein Ⅰ and Ⅱ was used in
the reaction mixture.The NR-inactivating proteins from wheat leaves also have inhibatory effect on notrate reductase activity from the roots. With polyacrylamid electrophoresis the more pure inactivating protein I was obtained with the molecular weight
of 47.5 kD. |
| Key words: nitrate reductase,inactivating protein,winter wheat |
