| 摘要: |
| 报道了用随机引物和混合分离分析（BSA)方法检测葡萄无核基因连锁的遗传标记。DNA混合样来自B72-216×B45-187的杂交后代。两个混合样分别由9个表现型为无核和有核杂种组成，除有核与无核性状外，其他性状是随机的。用10碱基随机序列的100个引物筛选混合样以期获得多态型DNA片段。结果在引物UBC-269扩增后，500对碱基的DNA多态型片段出现在无核样，而有核样不出现该多态型片段。进一步用杂种、父本及其无核基因的供给者无核白（Thompson Seedless）和百乐葡萄（Perlette）作模板，用引物UBC-269扩增，一些杂种、亲本及无核基因的供给者也拥有500对碱基的多态型片段。据此分析，葡萄的无核性是由多基因控制的，这些基因有主效和微效之分；本研究获得的分子标记U BC-269500与葡萄无核基因之一有连锁关系，而且与主效基因之一有连锁。 |
| 关键词: BSA，遗传标记，葡萄无核基因，RAPD，葡萄 |
| DOI： |
| 分类号:S663.103.2 |
| 基金项目: |
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| Identification of Genetic Marker Linked to Seedless Genes in Grapes Using RAPD |
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Wang Y J 1 Lamikanra O 2 Lu J 2 Ramming D W 3,Wang Y J 1 Lamikanra O 2 Lu J 2 Ramming D W 3,Wang Y J 1 Lamikanra O 2 Lu J 2 Ramming D W 3
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| Abstract: |
| This paper reports the rapid detection of a genetic marker linked to seedless genes in segregant populations of grapes using random primers and BSA (bulked segregant analysis). Two bulked DNA sampleswere generated from cross combination of B72-216×B45-187. One bulk containsm ixed DNA samples of 9 individuals that are identical for the seedlessness trait in phenotype, and anotherbulk are those of 9 individuals of the same family with the seeded trait, but arbitrary at all unlinked regions. The bulkswere first screened for polymorphism using a set of 100 arbitrary 10-mers primers. Only one polymorphism in the seedless traitpooldistinguished two bulks by primer UBC-269. The size of this polymorphism is 500 bp. The polymorphism was further featured in some hybrids and the pollen parent B45-187 as well as initial donors of seedless genes, Thompson seedless, and Perlette,when they were used as the templates for amplified by UBC-269. Based on the presence or absence of this polymorphism, segregation that can bemapped in the population occurs. Our results suggested that the seedlessness of grapes is controlled bymultigenes, includingmajor and m inor genes; The marker, UBC-269500 obtained in the study, was linked to one of the seedless genes in grapes. and it appears to be related to amajor seedless gene. |
| Key words: BSA,genetic marker,grape seedless genes,RAPD,vitis |
