| 摘要: |
| 综述了利用现代生物技术，即同功酶分析、线粒体ＤＮＡＲＦＬＰ分析以及ＲＡＰＤ－ＰＣＲ判别等进行蚜虫种下分类研究的概况与进展，比较了这几种方法在蚜虫种下分类研究中的优缺点。作者认为，同功酶法分析的是基因表达的产物，进行种下分类有局限性，需大量筛选最合适的酶类；ＲＦＬＰ、ＲＡＰＤ方法较准确，但需筛选大量限制性内切酶和随机引物。 |
| 关键词: 蚜虫种下分类，同功酶，线粒体，限制性片段长度多态性，随机扩增多聚酶链反应 |
| DOI： |
| 分类号:Q969.357.2 |
| 基金项目:国家自然科学基金资助项目 |
|
| A Review of Modern Biotechnique and Infraspecific Classification of Aphids |
|
Zhao Huiyuan Yuan Feng Zhang Gaisheng
|
| Abstract: |
| The general situations and the advances of scientific researches on aphid infraspecific classification by means of the modern biotechnique,including isozyme analysis , RFLP of mitochondrial DNA and RAPD-PCR ,are reviewed.The advantage and disadvantage of the three methods in the research of the aphid infraspecific classification are compared.Because there are limitations in the analysis of isozyme,a great quantitity of appropriate isozymes should be selecteThe method of RFLP and RAPD is accurate, but the RAPD is even simpler and time-saving. |
| Key words: Aphid,infraspecific classification,isozyme,mtDNA,RFLP, RAPD-PCR |
