| 摘要: |
| 酶消化配合机械分离，采集１０日龄小鼠腔前卵泡中的卵母细胞一颗粒细胞复合作（ＯＧＣs），以自制的胶原膜为底物，在 ＭＥＭ培养液中培养 １０ ｄ．卵母细胞－颗粒细胞复合体由 １１０． ５±１８． ６ μｍ增长到 １８４．４±６０． ６ μｍ，卵母细胞由 ５６． ２５±２． ０ μｍ增长到 ８２． ０±１０． ５μｍ．３６．７％的ＯＧＣs具有５层以上颗粒细胞，其中９．１％放出第一极体。 |
| 关键词: 小鼠,腔前卵泡,卵母细胞-颗粒细胞复合体,体外成熟 |
| DOI： |
| 分类号:S814.6 |
| 基金项目:国家自然科学基金资助项目和农业部生物技术重点项目 |
|
| Isolation and in Vitro Maturation of Mouse Oocytes from Preantral Follicles |
|
Zhang Yong, LI Yong, Li Yuqiang, Rui Rong,Zhang Yong, LI Yong, Li Yuqiang, Rui Rong,Zhang Yong, LI Yong, Li Yuqiang, Rui Rong
|
| Abstract: |
| The method of enzymatic digestion cooperating with mechanical isolation
for 15 minutes was used to collect oocyte-granulosa cell complexes (OGCs) from preantral follocles of 10-day-old mice.The OGCs were cultured in MEM medium on selfmade collagen membranes for 10 days.The diameter of OGCs increased from 110.5±18.6μm to 184.4±60.6μm,the diameter of oocytes increased from 56.25±2.0μm to
82.0±10.5μm.36.7% of oocytes gets 5 or more layers of granulosa cells,9.1% of
which were extruded a polar body. |
| Key words: mouse, preantral follicles, oocyte-granulosa cell complexes, in vitro maturation |
