| 摘要: |
| 应用光、电镜技术研究山羊排卵后和体外成熟培养后的卵母细胞的形态学变化。结果表明：排卵后４ｈ的输卵管卵母细胞和体外培养２４～２６ｈ的卵泡卵母细胞已具有充分成熟（获能）的形态学特征，卵周隙大，微绒毛竖起，ＭⅡ纺锤体长轴在质膜下呈垂直状，最大数量的皮质颗粒呈线形排列在质膜下，线粒体散在分布。本研究结果为山羊卵母细胞体外成熟和体外受精最佳时期提供了可靠的形态学依据。 |
| 关键词: 卵母细胞，超微结构，山羊 |
| DOI： |
| 分类号:S827.35 |
| 基金项目:陕西省自然科学基金资助项目 |
|
| Study on the Ultrastructure of Goat Oocyte Maturation |
|
Qian Jufen Liu Ling,Qian Jufen Liu Ling
|
| Abstract: |
| Morphological changes of the oocytes collected from oviduct after ovulation and those cultured in vitro for certain time were studied by using light and eledtron
microscopes.The study revealed that the oocytes after 4h of ovulation and those cultured
for 24～26h in vitro had the morphological characteristics of full maturation (capacitation),viz.large perivitelline space,upright microvilli,vertical MⅡ spindle axis and linear
cortical granules under cytoplasm membrance and dispersive mitochodrials.The results
would help determine the proper time for in vitro matutation and fertilization of goatoocytes. |
| Key words: oocyte maturation ,ultrastructure ,goat |
