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小麦条锈病罹病叶片核酸水解酶活力的变化
张 慧1, 商鸿生1, 李振岐1
西北农业大学植保系
摘要:
小麦初生叶接种条锈菌后,亲和反应寄主叶片RNase活性,分别在潜育期和产孢期呈双峰型增长;不亲和反应叶片RNase活性在侵染初期高于健康对照,但低于亲和性反应,其后随着病程发展,近免疫反应叶片RNase活性与对照相同,而中度抗病反应叶片也呈双峰增长,峰高及峰延续时间不同于亲和性反应。条锈菌侵染对DNase活性影响较小,亲和反应叶片仅在产孢阶段DNase活性有所增强,中度抗病反应叶片在显症时活性增强。
关键词:  小麦条锈病,核糖核酸酶,脱氧核糖核酸酶,核酸代谢
DOI:
分类号:S435.121.4
基金项目:高等学校博士点基金资助项目
Activity of RNase and DNase Healthy and Stripe-rust Infected Wheat Leaves
ZHang Hui Shang Hongsheng Li Zhenqi
Abstract:
Nucleic acid metabolism of wheat stripe rust inflected plants was systematically studied in 1991-1994,which showed that the increse of RNase activity in compatible primary leaves of wheat(Triticum aestivum)during the infection process ofstripe rust(Puccinia striiformis West)followed a twin-peak model with the one in thelatent period and another in the sporulation period.In infected incompatible host leavesthe RNase activity was higher than that in healthy leaves and lower than that in infected compatible host leaves in the early stage of infection process afterward which decreased to the same leval of the healthy leaves in near-immune host while the RNase activity inmoderate resistant host leaves increased in the form of twin peaks with different peak height and lasting time from that in the compatible. In addition,it was found that the effect of stripe rust infection was weaker on DNase than on RNase activity. Compatible only in sporulation period and moderate resistant host leaves in symptom-appearance period shovved increase in DNase activity respectively.
Key words:  wheat stripe rust(Puccinia striiformis West),RNase,DNase,Nucleic acid metabolism