| 摘要: |
| 用5％二甲基亚砜＋10％葡萄糖或5％二甲基亚砜＋10％蔗糖作为冰冻保护剂，能有效减轻中华猕猴桃愈伤组织细胞的超低温伤害。以－1℃／ｍｉｎ的速率降温至－60～－75℃时，对愈伤组织细胞的伤害作用最小；以大于160℃／ｍｉｎ速率快速化冻，愈伤组织细胞的相对存活率最高。愈伤组织细胞伤害的主要温度区域为－20～－40℃；－60～－75℃为愈伤组织细胞投入液氮时的“安全温度区”，－60℃和－75℃分别为“临界上限温度”和“临界下限温度”。 |
| 关键词: 中华猕猴桃，愈伤组织细胞，超低温保存，液氮，种质保存 |
| DOI： |
| 分类号:S129 |
| 基金项目:国家自然科学基金资助项目 |
|
| The Injury of Actinidia chinensis Callus Cells in Super-low Temperature |
|
Guo Yanping Li Jiarui,Guo Yanping Li Jiarui
|
| Abstract: |
| Being as cryoprotectants,5% DMSO-10% glucuse or 5% DMSO-10% sucrose could greatly reduce the injury of Actinidia chinensis callus cells.The injury of callus cells was minimal when freezed at the rate of -l℃/min.to -60～-70℃. The survival rate of the callus cells was maximal when rewarmed at the rate of more than 160℃/min -20～-40℃ was the major temperature scope of the callus cell injury;-60～-75℃ was the security temperature scope when callus cells were immersed in liquid nitrogen;-60℃ and -75℃ were the upper and lower limits of critical
temperatures respectively. |
| Key words: Actinidia chinensis,callus cell,super low temperature preservation,liquid nitrogen,germplasm preservation |
