| 摘要: |
| 以玉米穗粒腐病的主要高原菌串珠镰刀菌Fusarium moniliform Sheld为菌源，采用4种接种方法比较结果，以针刺果穗较深处（籽粒与穗轴间）发病最重，花丝喷雾法发病最轻；接种时期以乳熟期接种为好；接种浓度以10×40倍，每视野60个分生孢子接种效果最好；接种量以每果穗接种2.5ml孢子悬浮液发病重，效果好。 |
| 关键词: 玉米，穗粒腐病，接种技术，抗性鉴定 |
| DOI： |
| 分类号:S513.034 |
| 基金项目:陕西省科委资助课题 |
|
| Inoculating Technique of Corn Ear-Rot and Identifying Resistance Varieties |
|
Li Yaling Ma Blngyuan Li Duochuan Long Shusheng
|
| Abstract: |
| Four inoculating methods with Fusarium moniliform Sheld as the main
pathogens of corn ear-rot were compared.The results indieated that using needle puncturing corn ear produced the serious disease occurence,but the method of spraying inoculation to filaments caused lightest disease occurence.The optimal inoculation time was
at milk stage.Among three inoculation concentrations,the most effective inocuation was
obtained by concentration of 60 conidia in one field of vision under microscope at 10 X 40
times amplification.Comparative study of three amounts of inoculating,the sever disease
and better effect were obtained by inoculating 2.5mL conidial suspension to each corn
ears. |
| Key words: Corn ear-rot pathogens,inoculation technique,fesistant identification |
