| 摘要: |
| 利用限制性内切酶酶切和分子杂交等手段,对弗兰克氏菌菌株At4和Hr16的固氮基因克隆的nifH(pPC1201)、nifD(pPC1202)及nifK(pPC1203)基因为探针杂交,定位了At4和Hr16的nifH和nifD基因。同时发现,一条1.3kb的BamHI片段丢失。 |
| 关键词: 弗兰克氏菌 分子杂交 肺炎克氏杆菌 固氮基因 基因定位 转座子 亚克隆 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目 |
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| Mapping of NifH and NifD Genes of Frankia |
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Zeng Wangyong Cui Yuhai Bai Xueliang Ma Qingsheng
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| Abstract: |
| Nif gene clones from Fankia strain At4 and Hrl6 were analysed physically using digestion of restriction endonucleases, molecular hybridization and other techniques. And then, they were hybridized with nifH (pPC1201), nifD (pPC1202) and nifK (pPC1203) genes from Klebsiella pneumoniae, respectively. At the same time a loss of 1.3-kb BamHI DNA fragment was discovered. |
| Key words: Fankia molecular hybridization Klebsiella pneumoniae nitrogen fixation genes gene mapping transpon sub-clone |
