| 摘要: |
| 应用SDS-PAGE单向和双向电泳分析了籽瓜种子萌发过程中球蛋白组分的降解变化。55KD球蛋白在萌发开始后的前2天内降解最快,随后降解速度减慢,到子叶展平时完全降解。伴随着球蛋白降解,萌发第1天就出现30～31KD和34～37KD两种新组分,其含量在萌发的第5天增加至最大,随后又很快降解;这两种新组分都含有双硫键,其中34～37KD新组分可能是球蛋白的直接降解产物。球蛋白的酸性多肽降解快,碱性多肽降至解慢,它在萌发过程完成时依然存在。 |
| 关键词: 籽瓜,球蛋白,降解,α-酸性多肽,β-碱性多肽 |
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| 基金项目:国家自然科学基金资助项目 |
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| Changes in Degradation of Cucurbitin of Seedy Watermelon Seeds During Their Germination Stage |
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Zhang Yuxiu Zhao Wenming
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| Abstract: |
| The degradation of cucubitin of the seedy watermelon seeds was studied by one and two dimentional SDS Gel Electrophoresis during the process of their germination. Cucurbitin of 55KD was degraded most rapidly in the first 2 days of germination, later its degradation speed gradually slowed. When the leaflets spread, its degradation ceased. With its degradation going on, the new fractions of 30-31.KD and 34-37KD appeared in day 1 of germination, the contents of which increased most in the 5 th day of germination, and then rapidaly degraded. The two new fractions contained double sulfur bands, of which 34-37KD was considered to be a product of proteolysis of cucurbitin. The acidic polypetids of cucurbitin was rapidly degraded and basic polypetides was degraded slowly, and still existed when the process of germination completed. |
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