| 摘要: |
| 通过对冷冻液和平衡时间的筛选,研究了裸露小鼠卵泡卵母细胞的一步冷冻。结果表明:当冷冻液含25％的1,2—丙二醇和0.25mol/L的蔗糖,平衡时间为10min时,冷冻效果最为有效,解冻后存活率和形态正常率分别达66.2％和58.1％.解冻后形态正常的卵泡卵母细胞可以发育成熟,成熟率为22.7％。 |
| 关键词: 一步冷冻,卵泡卵母细胞,小鼠 |
| DOI： |
| 分类号: |
| 基金项目:国家教委博士点基金资助项目 |
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| One-step Freezing of Follicular Oocytes in Mouse |
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Hao Zhiming Qian Jufen Wang Jianchen
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| Abstract: |
| One-step freezing on denuded follicular oocytes in mouse was studied by selecting freezing solution and equilibration time.The results indicated that when the oocytes were placed in modified Dulbeccos phosphate buffered saline containing 25% 1,2-propandiol and 0.25 mol/L sucrose for 10 mimutes, freezing was the most efficient, and after unfreezing, the survial ang normal morphological rate reached 66.2% and 58.1%. However, the normal morphological oocytes after unfreezing were cultured in vitro, the maturation rate was 22.7%. |
| Key words: one-step freezing, follicular oocyte, mouse |
