| 摘要: |
| 经超滤,Con.A—Scpharose 4B亲和层析,从棉花黄萎病菌的Czapck′s培养液中,分离纯化致萎毒素。成分分析和致萎活性测定结果表明:棉花黄萎病菌产生的毒素是一种致萎力极强的糖蛋白。其含糖量为14.74％,蛋白质量为85.26％,其致萎活力是半纯化液的10～15倍,活力回收为76％。 |
| 关键词: 棉花,黄萎病菌,致萎毒素,糖蛋白,Con.A-Scpharose,4B亲和层析 |
| DOI： |
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| Isolation and Purification of Cotton Verticillium Wilt Pathogen and the Bioassay of wilting Phytotoxin |
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Can Li LQ Jindian
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| Abstract: |
| The wilting toxin was separated and purified from Czapck's medium liquid of cotton vcrlicillium wilt pathogen by ultrafiltration and Con.A-Scpharosc 4B affinity labeling. The component analysis and the results from bioassay of wilting phytotoxin showed that toxin was a kind of glyco-protein with a strong wilting ability, which contains 14.74% of carbonhydratc and 85.26% of protein .The toxin wilting activity was 12-15 times higher than that of half crude toxin , and the retraction wilting activity was 76%. |
| Key words: cotton,wilting toxin,verticillum wilting pathogen,Glyco-protein,Con.A-Sepharose 4B affinity labeling |
