| 摘要: |
| 采用改进的融合技术得到318个杂交瘤细胞系。经ELISA筛选,这些细胞系所分泌的抗体均和病毒抗原发生反应。亚克隆出26个杂交瘤细胞系,制出特异的单克隆抗体,并采用ELISA、放射免疫沉淀试验(RIPA)、SDS-PAGE、免疫印迹及病毒中和试验等对单克隆抗体的特性作了鉴定。应用单克隆抗体对牛疮瘆病毒Ⅰ型Bovine Herpes Virus I(BHV—Ⅰ)主要结构蛋白作了分析。 |
| 关键词: 传染性牛鼻气管炎病毒,单克隆抗体,SDS-PAGE,免疫印迹,病毒中和试验 |
| DOI： |
| 分类号: |
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| Preparation and Application of Monoclonal Antibodies of Infections Bovine Rhinofracheitis Virus(BHV-l) |
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Li jianqiang
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| Abstract: |
| 318 hybridoma cell lines have been obtained by using improved fusion method. These cell lines screened by ELISA are the secreted specific antibodies reacting to BHV-1 virus antigen. 26 hybridoma cell lines were cloned to produce specific monoclonal antibodies to the virus antigen. The characteristics of monoclonal antibodies were identified with ELISA, RlPA. SDS-PAGE, Immunoblot and Virus Neutralization test. The major BHV-1 structural peptides were analysed by monoclonal antibodies. |
| Key words: infectious bovine rhinotracheitis virus,Monoclonal antibody, SDS-PAGE, Immunoblot, Virus neutralization test |
