| 摘要: |
| 本文应用酶联 SPA 免疫吸附试验(ELISA)对710份奶山羊血清进行了布氏杆菌病的检测,并与 SAT 和 CFT 相对应。检测结果:ELISA 的检出率(62％,441/710)高于 SAT(60.56％,430/710,包括可疑反应)和 CFT(2.16％);漏检率(5.5％,39/710)低于 SAT(7.0％,50/710);经抗原处理的 ELISA 和 SAT 均呈阳性或阴性反应血清的 O.D 值,与原血清的 O.D 值相对比,前者差异显著(P<0.05),后者差异不显著(P>0.05)。说明 ELISA 是检测奶山羊布氏杆菌病的一种简单、特异、灵敏和快速的血清学方法之一,可用于本病的诊断检疫。 布氏杆菌病是一种人畜共患的慢性传染病,侵害多种家畜和动物,引起流产和不育等病症,影响畜牧业的发展,危害人民身体健康。目前常用的血清学试验方法有多种,各有其优缺点,但这些方法存在着敏感性低或因操作复杂而不易推广。因此,寻求简便、特异和敏感的检测法已为众望所归。用 ELISA 检测人、牛和猪的布氏杆菌病已有报道。现将我们应用酶联 SPA 免疫吸附试验检测奶山羊布氏杆菌病报道如下。 |
| 关键词: 免疫吸附试验,奶山羊,布氏杆菌病 |
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| Brucellosis in Milk Goats Detected by the Application of SPA ELISA |
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Zhang Guoxiang Li Jianqiang Wang Yinzhong Wang Yinfu Veterinary Science Department Northwestern Agricultural University
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| Abstract: |
| Brucellosis in sera of 710 samples of milk goats was detected by the application of SPA ELISA in comparison with SAT and CFT.Results from detection indicated that the detected rate(62%,441/710)of ELISA was higher than that(60.56%,430/710)including reaction in doubt)of SAT and CFT(2.16%);the undected rate(5.5%, 39/710)was lower than that of SAT(7.0%,50/710).ELISA and SAT treated with antigen appeared to have O.D value of positive or negative serum raetion/.In comparison with O.D value of the original sera,the former had an siginificant difference(P>0.05)while the latter had no siginificant difference (P>0.05).This indicated that ELISA was one of the simple,special,sensitive and fast serological methods in detecting Brucellouis in milk goats,and it can be used in diagnosis of quarantine. |
| Key words: ELISA,milk goat,Brucellosis |
